Kars Yöresi Koyunlarında Piyeten Olgularından Dichelobacter nodosus İzolasyonu ve PCR İle İdentifikasyonu
نویسندگان
چکیده
In this study it was aimed to isolation and identification by PCR of specific agent from ovine footrot in Kars district and thus determination of prevalence of disease. To this end, 8,970 sheep belong to 10 different flocks were examined clinically, and in 1532 of these (17.07%) were found lameness for various reasons. Out of 247 (2.75%) of these cases were evaluated to be footrot suspect clinically. Bacteria were isolated in 205 (82.99%) of the 247 samples that were cultured in an anaerobic environment due to the suspicion of footrot. When Gram stains and microscopic investigation was carried out on these isolates, 195 of them (95.12%) were found to be Gram negative rod-type bacteria. These isolates were subjected by polymerase chain reaction (PCR) using Dichelobacter nodosus specific primer and amplicons (440bp) of expected weight in 153 (78.46%) of isolates were found. Considering of these findings, it was concluded that prevalence of disease is high in sheep in Kars district.
منابع مشابه
Characterisation of Dichelobacter nodosus and detection of Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot.
The aim of this study was to determine the proportion of Dichelobacter nodosus, Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot compared to healthy sheep both at flock and individual level. The second aim was to characterise D. nodosus with respect to virulence, presence of intA gene and the serogroups. Swab samples (n=1000) from footrot-a...
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Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and differentiation of virulent and benign D. nodosus from interdigital skin swabs in a single test. Clinic...
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BACKGROUND Ovine footrot is a contagious disease with worldwide occurrence in sheep. The main causative agent is the fastidious bacterium Dichelobacter nodosus. In Scandinavia, footrot was first diagnosed in Sweden in 2004 and later also in Norway and Denmark. Clinical examination of sheep feet is fundamental to diagnosis of footrot, but D. nodosus should also be detected to confirm the diagnos...
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